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Polynucleotides
How to Inject Polynucleotides for Under Eyes
May 16, 2025
As an aesthetic professional, you’ve likely encountered clients interested in polynucleotides for under eye treatments. And it’s no surprise.
The skin around the eyes is often the first to show signs of aging and sun damage. It’s also one of the most delicate and technically demanding zones in aesthetic medicine, prone to dark circles, under-eye bags, crepey texture, and fine lines, yet highly sensitive to swelling and filler misplacement.
Polynucleotides offer a regenerative, low-risk alternative to traditional volumizing treatments. Instead of adding bulk, these DNA-based injectables stimulate tissue repair, boost hydration, and improve elasticity and overall skin quality.
With natural-looking results and minimal side effects, polynucleotides have become a go-to option for those seeking subtle, effective under-eye rejuvenation, without the downtime or risk of overcorrection.
Key Takeaways
- Due to biostimulating benefits and improved skin elasticity, hydration and pigmentation, as well as their ease of use, polynucleotide under eye treatments have become very popular among those patients wishing to tackle this facial area.
- Ideal polynucleotides under eye treatment candidates have fine lines, mild dark circles, or early signs of skin laxity, while contraindications include pregnancy, allergies to fish proteins, active infections, or being under 18.
- PNs eyes injections require an experienced professional, a precise technique using modern protocols and high quality product selection.
- Polynucleotide treatment downtime is minimal, with most patients experiencing only mild swelling or redness that typically resolves within a day or two.
About Polynucleotides
Polynucleotides, long chains of purified DNA fragments, often derived from salmon or trout, were initially developed for medical use in wound healing and tissue repair due to their regenerative properties.
Their transition into aesthetic medicine began around 2015, when their ability to stimulate fibroblast activity, boost collagen and elastin production, and enhance microcirculation gained recognition. Unlike hyaluronic acid (HA) injectables that add volume, PNs act as biostimulators, focusing on skin repair and rejuvenation. They effectively improve skin elasticity, hydration, and pigmentation, with a favorable safety profile and minimal downtime.
This has made them an increasingly popular choice in facial treatments, particularly for PN under-eye treatments.
Treating the Periorbital Area with Polynucleotides
DNA-based injectables work by supporting the skin’s natural repair process. This makes them a great choice, improving texture, softening fine lines, and gently brightening the area, while keeping results subtle and natural-looking.
Side effects are typically mild, making this treatment one of the easiest to undergo. Patients might experience slight swelling, redness, or minor bruising at the injection site, but these usually resolve within a few hours to a couple of days.
While periorbital PNs offer a subtle and natural improvement, it’s important to set realistic expectations. Results are not instant, as these treatments work by stimulating the skin’s own regenerative processes. Clients typically begin to notice visible changes, such as improved hydration, smoother texture, and reduced pigmentation, after a few weeks, with optimal outcomes developing after a full course of sessions.
Indications and Contraindications for Periorbital Polynucleotides
Before you begin the treatment with your client, make sure you are well acquainted with their medical history, as well as their expectations from the treatment.
That said, the PN under-eye treatment indications are:
- Fine line and wrinkle reduction with the help of collagen production stimulation
- Mild dark circle reduction
- Skin rehydration
- Mild to moderate skin laxity
- People with sun-damaged skin
- Anyone looking for a non-surgical alternative of an under-eye area rejuvenation
Even though these treatments are relatively risk-free, there are some PN treatment contraindications:
- Patients that are under the age of 18
- Patients that are pregnant or lactating
- Those with known allergies to fish proteins
- Those with active skin infections or irritations
It’s important to stress that PN treatments are animal-derived, and thus are not vegan-friendly.
Polynucleotide Injections vs. Under Eye Filler
While both PNs and dermal fillers are injectable treatments used to rejuvenate the eye area, they work in different ways.
HA injectables, like RESTYLANE®, are designed to add immediate volume, smoothing hollows and minimizing shadowing.
PNs, on the other hand, do not add volume. Instead, they stimulate the skin’s natural repair processes, encouraging collagen and elastin production, improving microcirculation, and enhancing hydration over time.
For certain patients, the best approach may even be to combine both treatments, using PNs to strengthen and prep the skin before introducing HA injectables at a later stage.
How to Inject Polynucleotides Under Eyes
Hopefully it goes without saying that polynucleotide treatments should only be conducted by licensed and experienced aesthetic professionals, using only quality products from reputable sellers, like Maylips via established protocols that will guarantee patient’s safety and satisfaction.
That said, the process of polynucleotide application should go as follows:
1. Consultation
This step includes verbal consultation that will cover the patient’s medical history, including allergies and sensitivity to any of the product’s ingredients. Then, the aesthetician should engage in a visual evaluation of the treatment area in good clinic lighting, determining the patient’s concerns and expectations of the procedure, and assessing possible contraindications, like an active skin infection.
2. Product Selection
Popular options include PN-based injectables like CROMA PhilArt EYE or PLINEST® Eye. Nucleofill eyes can also be considered. Final product selection is made based on the patient’s specific indications and desired results.
3. Preparation
This step includes cleansing the area with an antiseptic and a topical anesthetic application if needed. A medical professional should then select a 30-32 G needle or inject PNs with cannula.
4. Injection
Polynucleotide injections technique considers:
- Depth: injecting into superficial subdermal or deep dermal plane
- Technique options: microbulus or linear threading
- Needle entry point: 1-1.5 cm below the infraorbital rim
- Volume: 0.1 to 0.2 ml per side
- Massage: if needed, gently massage the area to disperse product
Injection should be slow and gentle, to reduce the possibility of bruising.
5. Aftercare
An aesthetician should offer cold compresses to help reduce swelling, and instruct their patient to avoid makeup, exercise and alcohol for the next 24 hours.
The treatment is generally well tolerated, and patients can go back to their usual daily routines afterwards. Downtime is minimal, and effect should appear gradually, with clients usually needing 2-4 sessions 2-3 weeks apart for the best results, with follow-up sessions every 6 to 12 months.
Final Thoughts
Polynucleotides offer a safe, effective, and natural-looking solution for under eye rejuvenation. By focusing on skin quality and regeneration, these DNA-based injectables help restore a fresher, more rested appearance without overfilling or distorting facial contours. As with any advanced aesthetic procedure, proper patient selection, high-quality products, and precise technique are key to successful outcomes.
By incorporating PNs into your treatment offering, you give your clients a natural way to refresh the eye area—with results they can see and confidence they can feel.
So, if you want to buy polynucleotides from reputable sellers, contact Maylips. With years of experience in the aesthetic industry, we aim to offer our clients top quality products at best prices available.
FAQ
- What are polynucleotides?
PNs are DNA fragments derived from salmon or trout that stimulate skin regeneration by boosting collagen, hydration, and tissue repair, used in products applied to skin via dermal injections.
- Do polynucleotides under eye injections help dark circles?
Polynucleotide under eye injections can help reduce mild dark circles by improving skin quality, hydration, and microcirculation.
- Polynucleotides under eyes vs filler: which is better?
PNs are better for improving skin quality and reducing dark circles naturally, while HA injectables are better for restoring lost volume under the eyes.
- Are nucleotides for under eyes safe?
PNs are considered safe when administered by a qualified professional, with minimal risk of side effects.
- What is polynucleotide for under eyes downtime?
Unlike with blepharoplasty and other surgical options, polynucleotide treatments have minimal downtime, with most patients returning to their daily routines shortly after the procedure.
References
- When to Use Tear Trough Filler Versus Polynucleotides | Harley Academy. Harleyacademy.com. Published 2024. Accessed May 13, 2025. https://www.harleyacademy.com/aesthetic-medicine-articles/when-to-use-tear-trough-filler-versus-polynucleotides/
- Alvin W, Chan L, Lee A, et al. Polynucleotides in Aesthetic Medicine: A Review of Current Practices and Perceived Effectiveness. International Journal of Molecular Sciences. 2024;25(15):8224-8224. doi:https://doi.org/10.3390/ijms25158224
- Team A. Innovative Uses of Polynucleotides in Aesthetic Medicine. Acquisition Aesthetics. Published July 18, 2024. Accessed May 14, 2025. https://www.acquisitionaesthetics.co.uk/blog/innovative-uses-of-polynucleotides-in-aesthetic-medicine/