TRH Thyrotropin 20mg

TRH (Thyrotropin-Releasing Hormone) 20 mg is supplied as a research-grade lyophilized powder intended for use in regulated laboratory environments only. TRH is also known as protirelin and consists of the sequence pyroglutamyl-histidyl-proline amide (pGlu–His–Pro–NH₂).

The 20 mg vial format supports laboratories conducting repeated assays, receptor-level signaling studies, and extended experimental workflows that require consistent peptide availability. Qualified laboratories that buy TRH peptide commonly employ it in neuroendocrine, autonomic, and central nervous system signaling research.

TRH (Thyrotropin-Releasing Hormone) is a compact hypothalamic neuropeptide endogenously produced by parvocellular neurons of the hypothalamus. It plays a central role in hypothalamic–pituitary communication and is distributed across multiple regions of the central nervous system, including the cerebellum, brainstem, and autonomic nuclei.

In laboratory research, the TRH peptide is widely used as a mechanistic probe for studying neuroendocrine signaling, G protein–coupled receptor (GPCR) activation, and second-messenger cascades. Its short signaling duration and well-defined receptor coupling make it a reference compound for both endocrine and neuroscience research models.

• Amino acid sequence: pGlu–His–Pro–NH₂ (also written Pyr–His–Pro)
• Peptide length: Tripeptide
• Molecular formula: C₁₆H₂₂N₆O₄
• Molecular weight: 362.39 g/mol
• PubChem CID: 638678
• CAS number: 24305-27-9

The TRH peptide binds to the TRH receptor (TRHR), a Gq/11-coupled GPCR expressed on multiple pituitary and neuronal cell types. Receptor activation stimulates phospholipase C (PLC), leading to the formation of inositol triphosphate (IP₃) and diacylglycerol (DAG).

These second messengers drive intracellular calcium release and activation of protein kinase C (PKC). Through this shared signaling cascade, TRH mediates:

• Thyrotropin (TSH) release from thyrotroph cells
• Prolactin release from lactotroph cells

This dual endocrine action explains why TRH is a valuable tool for studying multiple pituitary cell populations using a single, well-defined signaling mechanism.

TRH is supplied exclusively for laboratory research and is commonly used in:

1. Neuroendocrine signaling models: Hypothalamic–pituitary axis studies examining receptor sensitivity, hormone release kinetics, and feedback regulation.
2. Cerebellar synaptic plasticity research: Investigation of long-term depression (LTD), motor learning, and AMPA receptor trafficking in cerebellar circuits.
3. Autonomic network physiology: Studies of respiratory drive, cardiovascular regulation, and brainstem reflex integration.
4. Analog comparison studies: Direct comparison of TRH with stabilized analogs such as taltirelin to assess duration of action and resistance to enzymatic degradation.

All dosing values below reflect preclinical and in vitro research only:

• Intravenous injection: 0.1–40 μg
• Intravenous infusion: ~0.2 μg/hour
• Intraperitoneal (animal studies): 0.1–1.0 mg/kg
• In vitro assays: Nanomolar to micromolar ranges, assay-dependent

Observed effects include rapid calcium signaling, TSH and prolactin release, and modulation of neuronal excitability. Safe or effective human dosing has not been established.

When lyophilized or unopened:

• –80 °C: Preferred for 1+ year storage
• –20 °C: Up to 24 months
• 2–8 °C: Short-term use

Once reconstituted:

• Use sterile buffer at pH 7.2–7.4
• Store at 2–8 °C and use within 5–7 days
• May be aliquoted and frozen once at –20 °C for up to 3 months

Visual Inspection

• Clear solution is expected
• Cloudiness or discoloration may indicate degradation or contamination

Rapid degradation in biological fluids is expected due to enzymatic turnover. This rapid degradation is a key experimental consideration and explains why TRH is often compared with metabolically stabilized analogs in animal studies. Researchers must account for this property when designing dose timing, routes of administration, and the interpretation of transient signaling effects.

References

https://pubmed.ncbi.nlm.nih.gov/36147745/

https://pubmed.ncbi.nlm.nih.gov/26101376/

https://pubmed.ncbi.nlm.nih.gov/30618637/

https://pubmed.ncbi.nlm.nih.gov/9075462/

Compliance Notice

This product is intended for laboratory research use only and is not approved for human or veterinary use.